Sterilization of Microorganisms with Supercritical Carbon Dioxide
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چکیده
Thc sterilizing effect of supercritical carben dioxide (SC-CO,) is described as compared with gaseous C02(G-CO,) and liquid CO,(L-CO,). Baker's yeast, ltscherichia eoli, Staphylococcus aure us and eonidia'ofAspergiilus niger were sterilized by treating with SC-CO, at 200 atm and 350C when the water contcnt of each microorganism was 70 .90%, However, dry cells with a water content of 2 .1O% could not be sterilizcd undcr the same conditions. No sterilizing effect from SCC02 on endospores of Baeillus subtilis and B. stearothertnophilus was observed. G-C02 and L-C02 produced no sterilizing effect against both wet and dry baker's yeast cells, while wet E. coti cells were sterilized with G-CO,, Although dry conidia of A. niger could not be sterilized with SC-CO, alone, the addition of ethanol or acetic acid to SC-C02 made it possible to sterilize these, Without any decrease in their enzymatic activities, E. coli and baker's yeast in a-amylase and lipase preparations were sterilized with SC-CO,.
منابع مشابه
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تاریخ انتشار 2018